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You want to use pGLO to produce red fluorescent protein (RFP) instead of green fluorescent protein (GFP). To do so, you cut out the GFP gene from pGLO with two restriction enzymes,
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SOLVED: During the bacterial transformation lab; you come to know that the pGLO plasmid you transformed in the cells lacked the araC gene (figure below). What arc thc possible consequences of this?
1 KEY CONCEPTS: Genetic transformation, plasmid DNA, cloning, restriction enzymes, antibiotic selection, gene regulation, transc
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